Micropropagation is the rapid vegetative propagation of plants under in vitro conditions of high light intensity, controlled temperature, and a defined nutrient medium. Initiation, proliferation and development of micropropagation system for mass scale production of banana through meristem culture. Relative importance of location and period of banana bunch growth. Differences in dry matter, total soluble solids and citric acid concentrations in the pulp of ripe fruits were greater between periods than between sites. Serial removal of pseudo stem sheaths, using sterilized cutting tools, this should be concurrent with scarification of buds. Under bangladesh conditions, tissue culture derived plantlets of banana performed better than the conventional sword suckers faisal et al. Their size can vary and depends on the production objectives. Enterprising banana seedling producers could use iron rods and cast a concrete floor. Our laboratory has found that depending on the combination of culture vessel and gelling agent more economic methods can be chosen for successfully micropropagating banana. Carbon footprint of the banana supply chain food and agriculture. According to cyrus nyakundi they have a capacity to produce at least million banana tissue culture every year.
It is important that at least 50% shade is provided and that the fragile seedlings are wellprotected, by constructing a shade above the propagators. How i propagate tissue culture banana seedlings daily nation. Micropropagation of native cultivars of banana a critical. Aaa is propagated vegetatively and can be rapidly and efficiently propagated by micropropagation. The important factor affecting the efficiency of micropropagation of banana is the rate of invitro multiplication, due to different genomic compositions of cultivars hirimburegama and gamage. Cavendish types prepared in this way are particularly important in samoa. The fusarium wilt disease of banana, scientifically denominated as. The term carbon footprint is used to describe the quantity of greenhouse gases generated by an item or activity. Banana suckers selected are excised to obtain approximately 4 inches 10 cm of inner pseudostem tissue containing the banana meristem, as described in detail in fig. Pdf explants were taken from field grown plants in 2010. They are cultivated in areas of about 9 millions hectares spread throughout more than 100 countries, with an annual production of around 89 million metric tons fao, 2000. Bananasmusaspp, including plantains and cooking bananas, are among the most important horticultural crops in tropical and subtropical regions of the world. Content published by the market news service of cirad.
Micropropagation stages, types, applications, advantages, limitations. Collect at least two suckers from each plant source, one for micropropagation and the other for a nursery farm for future keiki needs. Micropropagation stages, types, applications, advantages. In vitro micropropagation of banana 647 as regards yield performance, tissue cultured plants have been reported to produce 39% higher yield than plants from sword suckers pradeep et al. One of the banana varieties that he produces is known as cavendish. Illustrated guide to the identification of banana varieties in. Joy and others published protocol for micropropagation of banana find, read and cite all the research you need on researchgate. Ppt banana tc 1 free download as powerpoint presentation. In this document, the general term banana is used to encompass cultivated varieties. Banana fusarium wilt tropical race 4 food and agriculture. Conventional micropropagation techniques, however, may be too costly for commercial purposes.
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