Radicalscavenging activity and ferric reducing ability of. Dpph 2, 2diphenyl1 picrylhydrazyl free radical scavenging assay dpph method is also used to. Freshly prepared dpph solution was taken in test tubes and extracts were added followed by serial dilutions 15. The inhibitory percentage of dpph was calculated according to the following equation. After 20 min incubation at room temperature, read the absorbance at 517 nm. Sanchezmoreno c 2002 methods used to evaluate the free radical scavenging activity in foods and biological systems. The ethanolic extract exhibited higher free radical scavenging effect than the water extract at all tested concentrations. Invitro free radical scavenging activity and bioavailability. Free radical scavenging activities of solutions of the plant extracts and synthetic antioxidant substances used in the study prepared in methanol at concentrations of 50, 100 and 200. The assay was carried out in buffered medium methanol.
Antioxidant activity by dpph assay of potential solutions to. The nitrite radical scavenging assay was carried out on the water extracts from a concentration range of 100 to. The crude extracts were diluted using ethanol according to the assay needs. Total phenolic and flavonoid contents were estimated using folinciocalteu reagent and aluminum chloride colorimetric assay methods, respectively. Evaluation of nitrite radical scavenging properties of.
Chapter vi evaluation of in vitro free radical scavenging. Characterization and dpph radical scavenging activity of. Antioxidant activity by dpph assay of potential solutions. Comparative study of abts radical scavenging activity and. Dpph radical scavenging activity pph radical is a stable organic free radical with adsorption band at 517 nm. The extract i mgml showed marked protection up to 66. Evaluation of antioxidant and free radical scavenging. Free radical scavenging activity, total phenolic content. Free radical scavenging in vitro and biological activity of diphenyl diselenideloaded nanocapsules. Free radical scavenging ability of the extracts was tested by dpph radical scavenging assay as described by blois and desmarchelier et al.
Dpph free radical scavenging activity of the extracts of the. In vitro nitric oxide scavenging activity of methanol. Free radical scavenging activity screening of medicinal. For assessing free radical scavenging potential of p.
In this assay, a molecule or antioxidant with weak ah bonding will react with a stable free radical dpph 2,2diphenyl1picrylhydrazyl. Free radical scavenging potential of picrorhiza kurrooa royle. Dpph free radical scavenging activity dpph is a purple colored stable free radical. Research article comparative invivo free radical scavenging. Dpph is a common abbreviation for the organic chemical compound 2,2diphenyl1picrylhydrazyl. Rapid assay highthroughput assay scavenging capacity index abstract a new microplateadapted dpph rapid assay was developed to assess the antioxidant capacity of pure compounds and foods. The determination of the total antioxidant activity frap assay in. Sixteen extracts showed strong antioxidant capabilities, which were, subjected for their dose dependent activity at different concentrations to calculate ic 50 values. Dpph radicalscavenging methods is common spectrophotometric procedures for. Ftir spectral studies clearly indicate the presence of various functional groups which may be attributed to the antioxidant and free radical scavenging properties of these extracts. Invitro antioxidant and free radical scavenging activity of.
Antioxidant and free radical scavenging activity of certain. Therefore, the search for naturally occurring antioxidants of plant origin is imperative. Antioxidant activities of the extracts were evaluated by 2,2diphenyl1picrylhydrazyl dpph free radicalscavenging ability, trolox equivalent. The freeradical scavenging activities of these compounds were tested by their ability to bleach the stable radical dpph. Dpph scavenging effect % a 0a 1a 0 x 100 where a 0 was the absorbance of the control reaction and a 1 was the absorbance in the presence of the sample of caffeine, caffeic acid and the combination. This rdsc assay is easy to perform and has acceptable accuracy 90. This assay uses this character to show herbs free radical scavenging activity. Free radical scavenging in vitro and biological activity. The antioxidant activity of the extracts was measured on the basis of the scavenging activity of the stable 1, 1 diphenyl 2picrylhyorazyl dpph free radical according to the method described by brandwilliams et al22 with slight modifications. The experiment has been performed in triplicate, was recorded as mean sd and their variance is analysed using oneway anova procedure as shown in table 1. Relationships between free radical scavenging and antioxidant. An assessment of the potential of proline to scavenge free radicals was made in a couple of in vitro assay systems, namely graft copolymerization and autooxidation of pyrogallol. Dpph free radical scavenging activity of the extracts of. A number of protocols have been followed for this assay resulting in variation in the results of different laboratories.
The free radical scavenging activity of methanol extract was measured by 1,1diphenyl2picrylhydrazyl dpph using the method of blois 1958. Dpph has two major applications, both in laboratory research. Antioxidant and free radical scavenging activity of spondias. Lower ec50 value indicates a higher dpph free radical scavenging activity.
Synthesis%2c spectroscopic%2c antibacterial and free. Department of pharmacy, gono bishwabidyalay, savar, dhaka 44, bangladesh. Total phenol and total flavonoid content in extracts, which reason antioxidant and free radical scavenging activity, was determined spectrophotometrically. The total phenolic content of the extracts was found to be higher in a. Pdf antioxidant and free radical scavenging activities. Antioxidant and free radical scavenging activities of some fruits article pdf available in journal of complementary and integrative medicine 81 january 2011 with 446 reads how we measure. Free radicalscavenging capacity, antioxidant activity and. Free radical scavenging activities of watersoluble extracts from some natural sources. Numerous attempts have been made to relate the free radical scavenging capacity of compounds to their antioxidant activity in foods even though antioxidant activity is dependent on both physical and chemical properties.
Dpph 1,1diphenyl2picrylhydrazyl is considered as a stable radical because. The extract showed total antioxidant activity with a trolox equivalent antioxidant concentration teac value of 0. Journal of chemical and pharmaceutical research, 2015, 77. Bioactive compounds, radical scavenging, antioxidant. Hence, the significant decrease in free radical can be attributed to the scavenging ability of methanolic extract of wattakaka volubilis leaves. The free radical scavenging activities of these compounds were tested by their ability to bleach the stable radical dpph. There are several assays to measure the antioxidant potential of compounds, the 1,1diphenyl2picrylhydrazine dpph radical scavenging assay being the most extensively used antioxidant assay for plant samples. Hydroxyl radical scavenging activity, nitric oxide radical scavenging activity, reducing power, lipid peroxidation inhibiting activity and total antioxidant assay using standard procedure. Triphala ethanolic extract exhibited potent free radical scavenging activity. An antioxidant is a molecule that inhibits the oxidation of other molecules.
Antioxidant activity and free radicalscavenging capacity. The aim of study is to compare the free radical scavenging activity of pineapple extract and eclipta alba extract by no assay. Evaluation of antioxidant and free radical scavenging capacities of polyphenolics from pods of caesalpinia pulcherrima. Low ic 50 values indicate high radical scavenging activity. The antioxidant assay scavenging prospects of 2,2diphenyl1picrylhydrazyl dpph radical the antioxidant activity of opdh2 and its synthesized metal complexes were determined using a stable 2,2diphenyl1picrylhydrazyl dpph reagent following a previous. The samples were reacted with the stable dpph radical in an ethanol solution. Dpph radical scavenging capacity of phenolic extracts from. Here, we aimed to investigate the antioxidant and free radical scavenging properties of methanolic extracts from tabebuia pallida t. Selective abts and dpph radical scavenging activity of. The ic 50 values for scavenging of free radicals were 112. The result of dpph free radical scavenging assay suggested that the extracts are capable of scavenging free radicals via electron or hydrogen donating mechanisms and. The publisher 2007 tutorial pdf free free radical scavenging activity of antioxidants in foods. The water extracts exhibited less free radical scavenging capacity than the ethanol extracts.
Radical scavenging and antioxidant activity of tannic acid. Antioxidant and free radical scavenging activity of. Invitro antioxidant and free radical scavenging activity. The measurement of the dpph radical scavenging activity was performed according to methodology described by brandwilliams et al. In vitro antioxidant and free radical scavenging activity. The differences between the free radical scavenging activity of laboratory and production. It loses this adsorption when accepting an electron or a free radical species, which results in a visually noticeable discoloration from purple to yellow 9. Antioxidant, dpph, free radical scavenging activity, medicinal plants, northeast india, tripura. In this paper, we report about the free radicalscavenging, nitritescavenging and nnitrosamine formation inhibitory activities of ethyl acetate extract eae and nbutanol extract be of c.
During the different stages of the brewing process the free radical scavenging activity is changed. Both these assays are essentially dependent upon free radical mechanisms. Free radical scavenging activity of ethanolic extracts from. Pdf antioxidant activity by dpph radical scavenging method. Ethanol extract of paederia foetida was mixed with 95% ethanol to prepare the stock solution 5 mgml. The aim of this work is to study and compare the antioxidant properties and phenolic contents of aqueous leaf extracts of juniperus thurifera, juniperus oxycedrus, juniperus phoenicea, and tetraclinis articulata from morocco. All the results were compared with same concentration of ascorbic acid as a standard which showed the highest free radical scavenging activity of 18. Wangf, yang qiua, baoyun sunc, gengmei xingc, jinquan dongc, xingjie lianga, chunying chena,c, adivision of nanomedicine and nanobiology, national center for nanoscience and technology. Antioxidant potential of the plant extract was measured in. Free radical scavenging potential of picrorhiza kurrooa. The dpph radical scavenging activity s% was calculated using the following equation. Antioxidants can scavenge free radicals and minimize their impact.
Scavenging of dpph free radical is the basis of a common antioxidant assay. A highthroughput relative 2,2diphenyl1picryhydrazyl dpph radical scavenging capacity rdsc assay was developed and validated in the present study. The hydrogen atom donating ability of the plant extractives was determined by the decolorization of methanol solution of 2,2diphenyl1picrylhydrazyl dpph. Free radical scavenging is associated with the antioxidant potential of a compound.
It is a darkcolored crystalline powder composed of stable freeradical molecules. The observed differences in free radicalscavenging capabilities support the hypothesis that both. In humans, many diseases are associated with the accumulation of free radicals. The free radical scavenging activity of the ethanolic extracts was carried out based on a method developed by re et al. The scavenging of reactive oxygen species and the potential for cell protection by functionalized fullerene materials junjie yinb,1, fang laoa,1, peter p. Dpph radical scavenging activity the free radical scavenging capacity of the extracts was determined using dpph. Dpph free radical scavenging activity of two extracts from. In the present study, the high dpph radical scavenging activity of the. In vitro antioxidant and free radical scavenging activity of. The scavenging of reactive oxygen species and the potential. A modified dpph assay was conducted to evaluate free radical scavenging activity using methanol extract of h. The dpph assay measures the ability of a compound to act as. The antioxidant activity using the dpph 1, 1diphenyl2picrylhydrazyl assay was assessed by the method of blois8. Dpph is a stable free radical that reacts with compounds able to donate a hydrogen atom.
The objective of this study was to compare the free radical scavenging activity of various compounds to their ability to inhibit lipid oxidation in foods. Pdf antioxidant activity by dpph radical scavenging. The hopping increases additionally the values of the parameter. Free radical scavenging activity of ethanolic extracts. In vitro nitric oxide scavenging activity of methanol extracts of three bangladeshi medicinal plants rozina parul1, sukalayan kumar kundu 2 and pijush saha2 1. Highthroughput relative dpph radical scavenging capacity. The result of dpph free radical scavenging assay suggested that the extracts are capable of scavenging free radicals via. Above 100gml, the ethanolic extract showed 80% scavenging activity, similar to control antioxidant compounds quercetin, rutin and lascorbic acid. Materials and methods dpph free radical scavenging activity processing of plants for extract preparation. Free radical scavenging activity of crude extracts and 4. Ftir spectral studies clearly indicate the presence of various functional groups which may be attributed to the antioxidant and free radical scavenging properties of.
What is the best method for radical scavenging assay. Dpph free radical scavenging capacity of legume extracts was evaluated according to the method of chen and ho 11 as modified by xu and chang 10. The antioxidant and free radical scavenging activity of the ethanol extracts of melia dubia hiern. It is a darkcolored crystalline powder composed of stable free radical molecules. Radical scavenging and antioxidant activity of ethanolic. We present a perspective of the protocols followed by different workers with incongruity in their results and recommend a standard procedure within. The antioxidant activity of the memq was evaluated by the phosphomolybdenum method according to the procedure of prieto et al. Dpph free radical scavenging activity of scopoletin also increased with increasing concentrations r2. The antioxidant and free radical scavenging activity were determined by several standard methods using spectrophotomer.
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